COMPARATIVE ANTIMICROBIAL ANALYSIS OF HERBAL AND NON-HERBAL ENDODONTIC IRRIGANTS USING PASSIVE ULTRASONIC IRRIGATION- AN IN VITRO STUDY
*Dr. Niveditha B., Dr. Veerendra Patil, Dr. Ratnakar P., Dr. Surabhi R. and Dr. Ashwini Marurkar
Root canal irrigants are used during chemico mechanical procedures as antimicrobial agents, debris removal,
lubricate and dissolve organic compounds. Several irrigants, such as sodium hypochlorite (NaOCl), chlorhexidine
(CHX), hydrogen peroxide (H2O2), normal saline are used. In this study we use 3% NAOCL, 2% CHX, Ozonated
oil, Neem extract to check antimicrobial efficacy against E.faecalis. Aim: The aim was to compare antimicrobial
efficacy of herbal and non-herbal endodontic irrigants using ultrasonic irrigation. Material and method: 40
extracted human single rooted teeth will be used for this study and grouped as.
Group 1 – 3% NAOCL
Group 2 - 2% CHX
Group 3 - ozonated oil
Group 4 - neem extract.
Teeth will be decoronated to a standardised length of 16mm and they will be chemo-mechanically prepared upto
#30 k file and autoclaved before inoculation. Each sample will be inoculated with E.faecalis, and incubated at 37°c,
7 days. Following this teeth will be enlarged upto f3 protaper, each group will be irrigated with irrigant as
mentioned before using passive ultrasonic irrigation. Microbial samples from each tooth will be collected using
sterile paper point, number of viable E faecalis colonies will be counted as Colony Forming Units. Results:
Although statistically insignificant the study showed that Chlorhexidine 2% has better antimicrobial efficacy,but
none of the groups could completely eradicate E.Feacalis.
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