ANTIMICROBIAL SUSCEPTIBILITY OF SALMONELLA SEROTYPES ISOLATED FROM HUMAN IN WEST-AFRICA (BURKINA FASO, MALI AND NIGER).
Hadiza Bawa Ibrahim*, René Dembélé, Gertrude Bsadjo Tchamba, Isidore Juste O. Bonkoungou, Flabou Bougoudogo, Alfred S. Traoré and Nicolas Barro
Acute diarrhea in developing countries can be caused by a broad spectrum of enteric pathogens including Salmonella enterica. The aim of this study was to determine the profile diversity among Salmonella isolates and the distribution of resistant phenotypes in West-Africa (Burkina Faso, Mali and Niger). Strains were isolated by the conventional method from diarrheal stools patients. Strains were confirmed using Analytical Profile Index 20 Enteric and serotyped using specific antisera. The isolates were tested for antibacterial resistance using the agar diffusion method with fourteen commonly used antibiotics. The double-disk synergy test for confirmation of extended spectrum beta-lactamase activity was carried out by using amoxicillinâ€“clavulanate against cefotaxime, ceftriaxone or cefepime. A total of 131 Salmonella strains were collected from the three countries with different proportions: 37% (48/131) to Burkina Faso, 48 % (63/131) to Mali and 15% (20/131) to Niger. The antibiotic susceptibility to different serotypes tested recorded the high resistance of Salmonella Paratyphi B to chloramphenicol. Salmonella Enteritidis showed only resistance to tetracycline. Resistance was observed to imipenem with Salmonella Paratyphi B and Salmonella Paratyphi C. Salmonella serotypes resistance study showed that 96% (126/131) were resistant to one or several antibiotics and 20% (26/131) were multiresistant. Six percent 6% (8/131) of Salmonella enterica isolates were producers of extended spectrum beta-lactamase. This study highlight emergence of multiresistant Salmonella to antibiotics in West Africa. Salmonella surveillance must be making in place in these countries and other African countries to improve epidemiological analysis of strains.
Keywords: Salmonella serotypes, antimicrobial susceptibility, Burkina Faso, Mali, Niger.
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