DEVELOPMENT OF STABILITY-INDICATING HPTLC METHOD FOR TRIFLURIDINE
Mrinalini Damle* and Ankita Mahajan
Trifluridine is anantiviral agent widely used in the treatment of eye infection caused by Herpes simplex virus. The work involves the development of the stability-indicating HPTLC method for the determination of Trifluridine. A rapid and simple stability-indicating HPTLC method has been developed and validated according to ICHQ2(R1) guidelines. TLC aluminum plate precoated with silica gel 60F254 was used as a stationary phase. The solvent system used, consisted of methanol: chloroform(1.5 : 8.5 v/v). A compact band of the drug was observed at Rf0.42 ±0.02.Stress degradation studies were performed under various conditions viz. hydrolysis at different pH, oxidation, thermal and photolysis conditions as per ICH Q1A(R2) and Q1B guidelines. Densitometric scanning-integration was performed by CAMAG Scanner 3at 261 nm using winCATS Software.
Keywords: HPTLC, Degradation, stability-indicating, Trifluridine.
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