EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ISOLATION AND IDENTIFICATION OF GRAM POSITIVE BACTERIA FROM PET RABBIT FAECES

Aisha Saleem*, Irum Naureen, Sehrish Rauf, Zohaib Hassan and Kashif Aziz

ABSTRACT

Rabbit faeces consist of numerous species of bacteria comprising both gram positive and gram negative. These bacteria are actually present in the caecum microbiota that forms a complex ecosystem which plays an important role in maintaining the reliability of the rabbit’s digestive health. The present study was conducted to examine the occurrence of gram positive bacteria in rabbit faeces. From 35 isolates, 14 strains of gram positive bacteria were isolated on the basis of Gram Staining technique from the rabbit faeces and distinctly labeled from RFS-01 to RFS-14. Afterwards these strains were identified as Staphylococcus aureus, Lactobacilli and Enterococcus spp. The percentages in incidence were 35.71% for both Staphylococcus aureus and Lactobacilli. While the Enterococcus sp. percentage in present study was 28.57. By applying biochemical test and after culturing bacteria on selective agar the bacteria species were identified. The Staphylococci species named were culture on Phenol Red Mannitol Salt Agar supplemented with 7.5% NaCl. Lactobacilli isolated on MRS and Pfizer Selective Enterococcus Agar was confirmed the presence of Enterococcus. The biochemical tests that were performed in present study were Catalase test, Oxidase test and the Coagulase test. The results may contribute to the study about the presence of gram positive bacteria in the faeces of herbivorous animals as well as evaluate the chances of microbial transmission from pet rabbits.

Keywords: Caecum microbiota, Gram staining, Lactobacilli, Staphylococcus, Catalase, Gram positive bacteria.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database