THE EFFECT OF BLOOD CLOTTING EFFECTS IN SNAIL, ACUSTA DESPECTA SIEBOLDIANA

Man Kyu Huh* and Yong Lim

ABSTRACT

Snail, Acusta despecta sieboldiana (Pfeiffer) is a genus of family Bradybaenidae and a terrestrial pulmonate gastropod mollusk. We investigated the blood clotting activity from Acusta despecta sieboldiana. Adenosine diphosphate (ADP) is a platelet agonist that causes platelet shape change and aggregation. When the water extract was added with 40 mg/ml of extract, it had a blood clotting or cohesive effect of about 14%. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) are measured the time it takes for blood to clot. The mean of PT in the water, ethanol, and methanol extracts were 11.2 s, 11.6 s, and 11.7 s, respectively. The time was 32.1 seconds on the 5 mg/ml water extract. Ethanol extract was 33.9 seconds on same concentration and methanol extract was 33.9 seconds. The international sensitivity index (ISI) was 1.3 for three extracts of snail mucus. International normalized ratio (INR) decreased in the high concentration exposure of snail mucus. In this study, snail mucus extract can partially help blood clotting when the wound is low in the agricultural field, but it did not have a significant effect. As a result of this study, it is expected that snail mucus extract can be used for blood coagulation as a hemostatic agent.

Keywords: Snail, Acusta despecta sieboldiana, adenosine diphosphate (ADP), Activated Partial Thromboplastin Time (aPTT), Prothrombin Time (PT).