INVITRO PANCREATIC LIPASE, ALPHA AMYLASE, ?LPHA GLUCOSIDASE INHIBITORY ACTIVITY OF BERGAPTEN

V. Jyothi*, Leemol S., Rajeshwari M., Muni Sirisha S. and P. Praneetha

ABSTRACT

In the present study the furanocoumarin, Bergapten was studied for in-vitro alpha (α)-amylase, alpha (α)-glucosidase and pancreatic lipase inhibitory activities. The aim of this work is to evaluate the inhibitory activities of the phytochemical Bergapten at different concentrations. Diabetes mellitus is a clinical condition characterized by hyperglycemia in which an elevated amount of glucose circulates in the blood plasma. Alpha amylase and alpha glucosidase inhibitors are used to achieve greater control over hyperglycemia in type 2 diabetes mellitus. The present study intends to screen novel pancreatic lipase, alpha amylase and alpha glucosidase inhibitors from natural sources like plants in order to minimize the toxicity and side effects of the inhibitors currently used to control obesity and hyperglycemia. The phytochemical Bergapten exhibited significant α-amylase, α-glucosidase and pancreatic lipase inhibitory activities with an IC50 value 8.54μg/ml, 9.11μg/ml and 7.22μg/ml respectively and well compared with standard acarbose for alpha (α)-amylase and alpha (α)-glucosidase and orlistat for pancreatic lipase inhibitory activities respectively.

Keywords: Bergapten, alpha amylase, alpha glucosidase, pancreatic lipase.