DEVELOPMENT AND EVALUATION OF LOTION OF AMPHOTERICIN-B

Chirag Ghangas*, Dr. Dilip Agrawal, Mohit Khandelwal3, Dr. Rakesh Goyal and Mr. Ashok Kumar Sharma

ABSTRACT

The main aim of this innovative research was to develop an effective and safe topical drug delivery formulation (Lotion) of Amphotericin-B to reduce the dose concentration of the active drug, to improve patient compliance, to promote rare side effects and increase the local fast absorption and action. Amphotericin B (AmB) is a polyene macrolide class of antifungal agent and it is the drug of choice for systemic fungal infection, but unfortunately, oral bioavailability of this drug is negligible due to its low aqueous solubility. Methods: Lotion formulations of Amphotericin-B were prepared using cetyl alcohol, steric acid glycerine with different penetration enhancer with their different concentrations. Two different formulations of Amphotericin-B were prepared and evaluated with respect to their colour, viscosity parameter, determination of pH, drug content, in vitro drug release studies, antifungal studies, and stability studies. Results: FT-IR and DSC study results that there were no any significant interaction and reactions between the drug, excipients. All the formulations of Amphotericin-B show acceptable standard physical properties. The drug content and percentage yield were higher for F1 formulation among all formulation F1 shows better drug release. Stability study of the best formulation F1 (Coconut oil) shows that there was no difference in drug content and in vitro drug release studies. Conclusion: From the above observation results that this F1 formulation (Coconut oil) may be more encouraging topical substitute for the healing of fungal infections in the skin.

Keywords: Amphotericin-B, Lotion, Skin, solubility, Polene, Fungal, Stability study.