THERAPEUTIC DRUG MONITORING OF VORICONAZOLE IN HUMAN SERUM USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
Dr. Peter H. Tang*
A fast, simple, selective, and specific high-performance liquid chromatographic (HPLC) method for the measurement of voriconazole in human serum has been developed. Sample preparation involved a single dilution step, solvent extraction, protein precipitation, and ultra-centrifugation. A commercially available compound micafungin was used as internal standard. Voriconazole and micafungin were extracted from serum samples using methanol and analyzed on a Zorbax Eclipse Plus C18 column with fluorescence detection set at excitation and emission wavelengths of 254 and 372 nm, respectively. The calibration
curve was linear through the range of 0.2-10 μg/ml using a 0.1 ml sample volume. Both the within-run and between-run precisions were <5%. Accuracies ranged from 97 to 105%. The method has been applied to monitor and optimize voriconazole therapy in clinical practice.
Keywords: Voriconazole, HPLC, fluorescence.
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