UV SPECTROSCOPIC METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF LAMIVUDINE IN BULK DOSAGE FORM
A simple, accurate and cost efficient UV Spectrophotometric method has been developed and validated for various parameters as per ICH Guidelines for estimation of Lamivudine in bulk powder and tablet dosage form. Distilled water was selected as diluents for proposed method for quantification of Lamivudine in bulk and pharmaceutical dosage form. The Lamivudine showed maximum absorbance at 260nm using distilled water as diluent. The sensitivity of the method is good and also the linearity which is observedover wide concentration range of 2-10 μg/ml. Regression equation and correlationcoefficient values were found to be Y = 0.097x + 0.0012 and R2 = 0.999, respectively indicating that proposed method was linear. The accuracy method was determined by recovery studies. The recovery of the drug is well within the acceptance limits of 98-102%. The precision was determined by analyzing the samples of Lamivudine. The% RSD for precision was found to be below 2%. The low% RSD values obtained from the analysis indicated that the method was highly precise.
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