EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ARTIFACTS OF CLASSICAL HISTOLOGICAL TECHNIQUES AT THE TESTICULAR SECTIONS IN THE WISTAR RAT

*Patricia Z. Deh, Bi Y.E.B. Goulai, Gnaté F. Montéomo, Abou J.L. Okon, Désirée Q. Coulibaly, B. Doukouré, Mohenou I.J.M. Diomandé and Gnangoran V. Yao

ABSTRACT

The objective of this study was to identify the artifacts of classical methods of tissues study at the testicular sections in adult rat. 4 testes were taken from 2 adult Wistar rats at 12 weeks of age. Samples were taken under ether inhalation anesthesia. The gonads were fixed in 10% saline formalin and dehydrated in alcohol and then embedded in paraffin. The transverse sections made by a microtome were spread out on slides. The histologycal sections were colored by hematoxylin-eosin and then covered with coverslips before optical microscope analysis. Observation of the histological sections showed different types of degradation caused by the technique. These are the formation of artificial voids in the seminiferous tubules, the tearing of the tunica albuginea, the destruction of part both interstitial tissue and peritubular sheath of the seminiferous tubules. Dye deposits were also observed. From the collection of tissue samples to observation, there are many steps that are likely to generate alterations. Therefore, proper handling as well as prompt fixation and careful tissue processing will reduce these artifacts.

Keywords: Artifacts, Histological testicular sections, Optical microscope, Wistar rats.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database