ARTIFACTS OF CLASSICAL HISTOLOGICAL TECHNIQUES AT THE TESTICULAR SECTIONS IN THE WISTAR RAT

*Patricia Z. Deh, Bi Y.E.B. Goulai, Gnaté F. Montéomo, Abou J.L. Okon, Désirée Q. Coulibaly, B. Doukouré, Mohenou I.J.M. Diomandé and Gnangoran V. Yao

ABSTRACT

The objective of this study was to identify the artifacts of classical methods of tissues study at the testicular sections in adult rat. 4 testes were taken from 2 adult Wistar rats at 12 weeks of age. Samples were taken under ether inhalation anesthesia. The gonads were fixed in 10% saline formalin and dehydrated in alcohol and then embedded in paraffin. The transverse sections made by a microtome were spread out on slides. The histologycal sections were colored by hematoxylin-eosin and then covered with coverslips before optical microscope analysis. Observation of the histological sections showed different types of degradation caused by the technique. These are the formation of artificial voids in the seminiferous tubules, the tearing of the tunica albuginea, the destruction of part both interstitial tissue and peritubular sheath of the seminiferous tubules. Dye deposits were also observed. From the collection of tissue samples to observation, there are many steps that are likely to generate alterations. Therefore, proper handling as well as prompt fixation and careful tissue processing will reduce these artifacts.

Keywords: Artifacts, Histological testicular sections, Optical microscope, Wistar rats.