PRE-ANALYTICAL NON-CONFORMITIES OF THE KARYOTYPE

*Patricia Z. Deh, Bi Y.E.B. Goulai, Malika J.A. Dieth, Abou J.L. Okon, Jessica A.L. Koffi, Désirée Q. Coulibaly, B. Doukouré, Victor G. Yao, Isidore M.J.M. Diomandé

ABSTRACT

Purpose: Quality assurance applied to the three phases of karyotype examination namely pre-analytical, analytical and post-analytical allows reliable results to be provider. However, errors or non-conformities (NC) such as insufficient information on the analysis report were noted during the first phase. The objective of this work is to evaluate the pre-analytical non-conformities of the karyotype inherent in the analysis report at the Histology-Embryology- Cytogenetic laboratory of the University Hospital in Cocody-Abidjan. Material and methods: This was a retrospective and descriptive study that was carried out in the Histology-Embryology-Cytogenetic laboratory of the Cocody University Hospital. The non-conformities of the karyotype prescription bulletins from 2014 to 2019 were listed on a data collection sheet. These analysis reports came from various health facilities. Results: 12 types of NC were identified on 59 bulletins. The non-conformities of the bulletin relating to the identification of the patient concerned the absence of sex (18.64%), age (28.81%), profession (100%), place of residence (100%) and the phone number (100%). The last three were the most recurrent. The other non-conformities relating to the prescription that had a low percentage were the absence of the reason for the prescription (1.69%), the identification of the prescriber and the requesting Department (52.24%). In contrast, the absence of the date and time of the sample, the maternal age at conception, recent treatments likely to affect the quality of the examination as well as the family history was the most frequent (100%). Conclusion: The performance of this evaluation made it possible to detect non-conformities related to the reports that could have an effect on the transmission of the result and the quality of the examination. To remedy these errors, the laboratory should carry out preventive and corrective actions such as, the establishment of a model prescription bulletin for the karyotype and the sampling compliance criteria sheet to be offered to the requesting Departments.

Keywords: Karyotype, Pre-analytical phase, Non-conformities (NC), Quality assurance.