AN IN-VIVO ASSESSMENT ON THE HEPATOPROTECTIVE ACTIVITY OF ETHANOL EXTRACT OF COCCINIA GRANDIS

Mehnaz Ali*, Sayeeda Sultana, Anonto Gomes, Sabiha Rahman, Arifa Sultana, Jannatul Ferdous, Md. Rafat Tahsin, Jakir Ahmed Chowdhury, Shaila Kabir, Abu Asad Chowdhury, Tahmina Akter, Fahima Aktar, Md. Shah Amran

ABSTRACT

Background: The liver is a crucial detoxifying organ in the mammalian body that also performs a number of metabolic functions. A wide range of toxic substances and drugs have been linked to liver damage. Coccinia grandis have been used to treat a wide range of diseases. In this study, an in vivo assay was conducted to assess the hepatoprotective activity of C. grandis. Method: Fresh C. grandis was collected and dried. Ethanol extract of C. grandis was obtained by mixing 400g of the powder with 2400 ml of 100% ethanol, filtering and then evaporating the filtrate. Test animals were categorized into 14 groups for measuring different parameters to assess the hepatoprotective activity of C. grandis. Result: C. grandis significantly normalized body weight, SGOT, SGPT, ALP, creatinine, total cholesterol, LDL, triglycerides level in CCl4-induced hepatic toxicity containing rodents (p< 0.05). The plant extract also reversed the impaired level of proteins, such as ϒ-GT, SOD, MDA, CAT, LDH which are closely related to hepatic function. Conclusion: This study shows that different doses of C. grandis extracts give significant hepatoprotective activity compared with established marketed drugs. Therefore, more study should be conducted to detect the responsible mechanism to exert desired activity.

Keywords: Hepatotoxicity, SGOT, SGPT, rodent, Coccnia grandis, MDA, CCL4.