FIRST ORDER DERIVATIVE METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATROPINE SULFATE AND DEXAMETHASONE
Vaghasiya Vivek and Dr. Kashyap Raval*
Analysis of pharmaceutical product is very important as it concerned with quality of life. The objective of present work is to develop and validate First order Derivative UV Spectrophotometry method for estimation of Atropine sulfate and Dexamethasone in its combined Liquid dosage form. Estimation of Atropine sulfate and Dexamethasone were carried out at λmax 239.60 nm and 246.68 nm for First Order Derivative Spectrophotometry Method, Using Methanol as a solvent. Linearity of Atropine sulfate and Dexamethasone were found to be 100-350 μg/ml and 10-35 μg/ml respectively. UV Spectophometric method was validated as per ICH guideline Q2 (R1), for its accuracy, Precision, LOD & LOQ and the results were found to be satisfactory. Developed and validated method was found to be simple, accurate, economical, robust and reproducible & method can be successfully applied for routine QC analysis.
Keywords: Atropine sulfate, Dexamethasone, First order derivative spectrophotometry, Methanol.
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