A STUDY OF AEROBIC BACTERIAL AND FUNGAL ISOLATES OF CHRONIC SUPPURATIVE OTITIS MEDIA AT A TERTIARY CARE CENTRE IN HYDERABAD.

Dr. Chandra Lekha Penchala, Dr. Sunitha Palvai* and Dr. Jyothi Lakshmi G.

ABSTRACT

Background: Chronic suppurative otitis media (CSOM) is a major health problem causing serious local damage and threatening intra and extra cranial complications. Purpose of this study is to find the prevalence of various isolates and antibiotic susceptibility patterns in CSOM cases. Method: In 100 clinically diagnosed CSOM cases, 2 ear pus swabs from middle ear cavity were collected aseptically, one swab was used for Gram stain and KOH, another for inoculating Blood agar, Maconkey agar and Sabouraud’s dextrose agar, antibiotic susceptibility done on Muller Hinton agar by Kirby Bauer disc diffusion, multidrug resistance detected and phenotypic tests done for ESBL, MBL detection by combined disc synergy tests, Cefoxitin disc diffusion test was used to detect MRSA. Age, gender, educational and demographic data was collected. Result: Of the 103 samples processed from 100 cases, 6 were culture negative and 97 were culture positive, showing 92 bacterial and 10 fungal isolates overall, most predominant organism was Pseudomonas aeruginosa with 50.9% prevalence, of which 25 % were ESBL, 7.69 % were MBL producers, second most common being Staphylococcus aureus with 29.4% prevalence, of which 47% were MRSA . Rest were Proteus (5.8 %), Klebsiella (1.9%), Escherichia coli (1.9%), most of the isolates were sensitive to Amikacin . Aspergillus and candida species were isolated in fungal culture which was 9.8% of total isolates. Conclusion: Early intervention is key factor to prevent progression to complications. Periodic assessment of etiological agents, changing drug resistance patterns is essential.

Keywords: Chronic suppurative otitis media, Pseudomonas, Methicillin resistant Staphylococcus aureus.