DIETARY INTERVENTION OF DACRYODES EDULIS PULP OIL ON 3-METHYLCHOLANTHRENE INDUCED PROSTATE CARCINOGENESIS AND EXPRESSION OF COX-2 AND PPAR-? IN WISTAR RATS
Esosa Samuel Uhunmwangho* and Onoriode Oyiborhoro
Prostate cancer is the major cause of cancer death in man, and one of the factors that influence its occurrence is diet. The present study investigated the dietary intervention of feeding Dacryodes edulis (African Pear) pulp oil (DEPO) on 3-methylcholanthrene (MCA) induced prostate cancer in Wistar rats and expression of cyclooxygenase-2 (COX-2) and peroxisome proliferator activated receptor gamma (PPAR-γ) in the prostate gland. The pulp oil was extracted with n-hexane using Soxhlet apparatus and characterized by gas chromatography. A total of 52 male Wistar rats (28 days old) were used. Group-A was fed with diet containing 15% DEPO for 14 days before single intraperitoneal administration with MCA (250mg/kg). Afterwards, the animals were fed with DEPO for twelve weeks before sacrifice. Group-B animals were administered MCA (250m/kg) to induce prostate cancer, but was not fed with the DEPO, and group-C animals were fed with 10% DEPO only. In the MCA treated group, tumor latency period was 7weeks on animals fed with DEPO compare with those not fed with the DEPO diet which was 3weeks. The expression of cyclooxygenase-2 was significantly (P < 0.05) less, and PPAR-ɣ more on DEPO treated animals. The gas chromatography results showed that polyunsaturated fatty acids such as linolenic acid, eicosadienoic acid and docosadienoic acid were incorporated into the membrane of the prostate gland. This study showed the validity of Dacryodes edulis pulp oil in the management and treatment of prostate carcinogenesis.
Keywords: 3-methylcholanthrene, Prostate cancer, Dacryodes edulis, Cyclooxygenase-2.
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