GREEN CHROMATOGRAPHIC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF PARACETAMOL & LORNOXICAM USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) IN BULK

Shivangi*, Jain Neetesh Kumar and Soni Priyanka

ABSTRACT

A simple Green (eco-friendly), simple, selective, rapid, precise, reverse phase HPLC analytical method is proposed and validated for the simultaneous estimation of Lornoxicam and Paracetamol using environment friendly solvents. The result observed showed that ethanol: phosphate buffer at pH 3.0 adjusted with orthophosphoric acid in 35:65 ratios, at a flow rate of 1.0ml/min was identified as the best chromatographic method for simultaneous estimation of these drugs in line to green analytical separations. The method was developed on Thermostat hypersil C18 column (5 μm, 250mm x 4.60mm) as the stationary phase. The detection wavelength taken was 262.5nm. The method was validated in accordance with the ICH guidelines and validated for linearity, selectivity, LOC, LOQ and robustness. The result showed that the developed method is precise, accurate and can be applied for determination of the same drugs, for routine analysis with affecting the environment.

Keywords: Green chromatography, Lornoxicam, Paracetamol, Simultaneous estimation.