ANTICANCER POTENTIAL OF AEGLE MARMELOS BARK EXTRACT AGAINST DMBA INDUCED SKIN PAPILLOMAGENESIS WITH REFERENCE TO OXIDATIVE STRESS

Nirmala Gupta*, R C Agrawal, Pratima Sharma and Anita Narwariya

ABSTRACT

Background: Cancer is among the second fatal diseases, next to cardiovascular diseases. Surgery, chemotherapy and radiotherapy or a combination of these are used worldwide for the treatment of Cancer. But, all these therapeutic options results in various side effects, to overcome these side effects, medicinal plants and herbs have now been used for their pharmacological active contents to treat cancer. Aim: The present study was aimed at evaluating the Chemopreventive and Antioxidant effects of hydromathanolic Aegle marmelos bark extract against 7, 12-dimethylbenzeneanthracene (DMBA) induced Papilloma in Swiss albino mice. Material and Methods: Crude extraction protocol was employed for the preparation of Hydromethanolic Aegle marmelos bark extract and Tumor was induced by the application of single dose of DMBA on the dorsal, shaved surface of mice and promotion by croton oil started after 2 weeks and in the treatment group, A. marmelos bark extract (900 mg/kg b.wt.) was given one hour before each application of croton oil 2 times/week up to 16 weeks. After the completion of the assay, Reduced Glutathione (GSH) level was estimated in control, Carcinogen control and treatment group. Results: In this Skin Papilloma model, Significant reduction in tumor burden, tumor incidence and cumulative number of papillomas and a marked increase in average latent period of tumor appearance and Reduced Gluatathione concentration was observed in Aegle marmelos bark extract treated group as compared to animals treated with DMBA and croton oil (carcinogen control) group. Thus, the present study suggests that the Aegle marmelos bark extract has antitumor and antioxidant potential against Chemical induced skin papillomagenesis.

Keywords: Anticancer, Chemopreventive, Antioxidant, Hydromethanolic, Aegle marmelos, Papillomagenesis.