EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

NELUMBO NUCIFERA (LOTUS) LEAF EXTRACTS IN VITRO ANTICOAGULANT ACTIVITY ON NORMAL HEALTHY BLOOD PLASMA

*Dr. Nabeela M. Khan, Dr. Pankaj Surve and Dr. Aparna Ghotankar

ABSTRACT

Formation of clots within the walls of damaged blood vessels is called haemostasis. To maintain intravascular blood in a fluid state and to prevent abnormal bleeding , in this study we aimed to evaluate the possible anticoagulant effect of leaf extracts of Nelumbo nucifera. Materials and Methods: For in vitro prothrombin time(PT) The aqueous, methanol, acetone, and ethyl acetate extracts of N. nucifera at different concentrations were tested. In different concentrations 0.5, 0.25, 0.125, and 0.062 g/ml. The invitro anticoagulant effect of different extracts of N nucifera were examined using plasma, from blood samples of normal individuals by measuring PT. Ethylenediaminetetraacetic acid (EDTA) and saline in distilled water were used as a negative and positive control, respectively. The extract plasma was subjected to anticoagulation activity and was compared with EDTA-plasma and saline plasma. Results: The methanol leaf extract of N. nucifera was found to inhibit coagulation process in 60 min: 3 s in 0.5 g/ml. The time taken for clotting at the concentration of 0.5 g/methanol leaf extract showed the moderate effect of 10 min:20 s with respect to control while ethyl acetate extract showed the least effect of 8 min: 23 s compared to control. Overall, the concentration of 0.5 g/ml of leaf extract showed a maximum effect in all the tested extracts with respect to other concentrations 0.25, 0.125, and 0.062 g/ml. Thus, N. nucifera methanol, ethanol, and ethyl acetate leaf extract in different concentrations inhibits clot formation and increases the PT in a dose-dependent manner. Due to the presence of certain phytochemical constituents in the crude extract prolonged prothrombin activity could be observed. Phytochemical analysis revealed the presence of tannins, flavonoids and steroid compounds in the crude extract and further, the active principles could be isolated and evaluated for clinical or physiological purposes. Conclusion: In vitro, anticoagulant activity studies results demonstrated that leaf extract N. nucifera possesses pharmacologically active anticoagulant components which could be helpful in preventing blood clotting disorders. Thus, as a supplementary source of natural anticoagulant in future N nucifera leaf could be used.

Keywords: Hemostasis, phytochemical, prothrombin time, clotting factors, calcium.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )