ANALYSIS BY HPLC OF THE CHLOROFORMIC EXTRACTRESULTING FROM SEEDS OF ALYSICARPUS OVALIFOLIUS (SCH. AND TH.) TESTED ON LARVAE OF MOSQUITO.

Dr. Alioune Ndiaye*, Dr. Nicole Dossou, Dr. El Hadji Gorgui Diouf and Pr. Moussoukhoye Diop

ABSTRACT

Introduction: Alysicarpus is a kind of plants with flowers belonging to the family of Fabaceae, made up of 106 species.[1] The Alysicarpus kind is resistant to the drought, tolerating with salinity and resistant to the diseases and the ravagers. These properties are associated with their secondary wealth of metabolites such as the flavonoids, the alkaloids tannins etc. These secondary metabolites currently constitute tracks of made up of origin vegetable used like repulsive or toxic products against insects and ravagers. One can set the example of rotenone, rotenoïdes and pyrethrins.[2] Indeed, In the fight anti-mosquito, the active matters of insecticides of synthesis used present several disadvantages. To ensure a better intervention, while preserving to the maximum the natural environment, of new preventive methods as of new products are constantly required.[3] The family of the leguminous plants whose been part the Alysicarpus kind offers a panoply of capable compounds to help to fight against certain insects like the mosquitos. The latter are responsible for diseases like paludism. This disease remains public health problems in Senegal because the burden of the disease is always heavy in particular in certain areas of the country and the deaths which are allotted to him persist.[4] The important place that the mosquitos in the terrestrial environment as in the aquatic environment occupy on the one hand, and the fight against the diseases transmitted by their punctures on the other hand, make these Arthropods an important equipment of study for the biologists.[5] Then, the natural substances with bioactives molecules resulting from the plants currently arouse a very particular interest by their multiple biological activities (antibacterial, antioxydant and insecticidal).[5] It is in this context that this study on Alysicarpus ovalifolius (Sch is registered. and Th.) which, to our knowledge, was not yet the object of studies pushed in this direction. With an aim of filling this gap, this study presents the bioactivity and HPLC of the Chloroformic fraction of Alysicarpus ovalifolius analyzes tested on larvae of mosquito.

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