HPLC ANALYSIS OF FRACTIONS FROM CHLOROFORMIC EXTRACT OF Indigofera pilosa (PEAR) LEAVES TESTED ON MOSQUITO LARVAES

Alioune Ndiaye*, El Hadji Gorgui Diouf, Nicole Dossou and Moussoukhoye Diop

ABSTRACT

Indigofera alone represents more than 700 species in tropical and subtropical regions of Africa, Asia, Australia, South and North America.[1] Indigofera, apart from their predominant roles in animal fodder, and in the textile industry, are now an important source of secondary metabolites (flavonoids, rotenoids and phenol acids).[2] Based on the biological properties of each of the compounds identified or characterized (rotenoids, terpenoids, sterols, saponosides, isoflavonoids) in the genus Indigofera,it would be important to study one of the species of thisgenus: Indigofera pilosa (Poir) and compare it, in terms of its content, to other species of the same family. In addition, secondary metabolites characterized in the genus are currently tracks of plant-based compounds used as repellents or toxic products against insects and pests.[3] Indeed, researchers are currently concerned about their harmful role in diseases such as malaria and crop destruction. Also, the active ingredients of synthetic insecticides used have several drawbacks. To ensure better intervention, while preserving the natural environment as much as possible, new preventive methods and new products are constantly sought.[4] It is in these contexts that this study on Indigofera pilosa, which has been the subject of little literature, takes place. This study will focus on bioactivity on mosquito larvae and HPLC-UV analysis of fractions from the chloroformic extract of the leaves of this plant.

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