ASSOCIATION BETWEEN COMPLEMENT COMPLEX C5B-9 WITH RHEUMATOID ARTHRITIS IN AN IRAQI POPULATION
Ghadaq Hameed Neamaa* and Amjed Mohammed Sahib
Purpose: Investigating the level of concentration of complement complex C5b-9 in RA patients and controls serum. Methods: allow 2 ml blood samples to clot for 2 hours at room temperature or overnight at 4 C after that the samples were centrifuged for 15 min at 1000xg. Then, the the supernatant was collected and separated in plastic plain tube at deep freeze (-20 C˚) to be used for determine the concentration of complement complex C5b-9 in the serum of cases and controls. The complement complex C5b-9 concentration levels were investigated in 55 RA patients and in 32 healthy controls by using ELISA test. Results: The complement complex C5b-9 concentration levels were investigated in 55 RA patients and in 32 healthy controls. There is no significant difference when the mean of concentration level is compared in patients sera with the mean of concentration level in control sera. When the complement complex is used as a diagnostic test according to ROC curve, it appeared not beneficial for the diagnosis of RA when compared with Rheumatoid Factor (RF) and ACCPs (Anti Cyclic Citrullinated Peptide antibodies). Conclusion: there were many laboratory diagnostic tests to the diagnosis of RA like CRP, RF and ACCP but the ACCP antibodies test was more specific for predicting and/or diagnosing RA. and the Complement complex C5b-9 was not beneficial for prediction the diagnosis of RA. there is a significant difference when the mean of complement complex is compared according to positive and negative RF test among cases with RA.
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