EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

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 ICV - 79.57

Abstract

COMPARATIVE ANALYSIS OF SYPHILIS SCREENING TECHNIQUES IN A POPULATION OF PREGNANT WOMEN

Salma Hasnane*, Anass Jarmoumi, Mustapha Deqaq, Chaimaa Benlabsir, Jalila El Bakkouri and Hassan Fellah

ABSTRACT

Syphilis is a Sexually Transmitted Infection, due to T. pallidum, it has increased considerably since the 2000s. Consequently, it represents a major activity for biological analysis laboratories. VDRL-charcoal and TPHA are the two main screening and serodiagnosis tests for syphilis. In the present work, we carried out a cross-sectional, monocentric study focusing on the search for anti-treponemal antibodies in serum samples from pregnant women consulting at the peripheral hospital in Casablanca, using four distinct syphilitic serology kits, in order to evaluate two kits from the firm PLASMATEC® (VDRL-charcoal and TPHA), compared to the kits used routinely and therefore that we considered as a reference by the laboratory of this peripheral hospital, this concerns the kits from the company BioSystems® for the VDRL-charcoal test and from the company OMEGA® for the TPHA test, with a the possibility of introducing them into routine analysis. The sample consisting of 100 sera from pregnant women was tested by the VDRL-charcoal and TPHA tests technique using the BioSystems® reference kit (RPR-charcoal) and the reference kit OMEGA® (TPHA), and the tested PLASMATEC® kit (VDRL-charcoal, TPHA). Both kits gave negative results for all samples analyzed, with 100% correlation, 0% sensitivity, 100% specificity, and 0% and 100% PPV and NPV, respectively. Despite the 100% correlation of the results of the kits tested against the reference kits. Their possible introduction to routine use remains subject to carrying out studies with samples that are more representative in number and quality (positive sera, sera from patients with confirmed clinical signs of syphilis).

Keywords: VDRL-charcoal and TPHA.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia