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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



Safoora Ishfaq, Syed Dilnawaz Ahmed, Asad Hussain Shah, Rizwan Taj Khan, Syeda Maria Fiaz Bukhari, Isma Hameed, Hira Mubeen, Nazish Awan, Syed Rizwan Abbas and Shahid Raza*


The experiment was conducted to check the sterilization effect of different concentrations of Clorox on six wheat varieties. This experiment was conducted in plant tissue culture lab of biotechnology, university of AJK. Seeds of selected wheat cultivars were used as an explant and surface sterilized with different (30%, 50% and &70%) concentrations of Clorox. After sterilization they were washed three times with double distilled water for 10 minutes. Different concentrations of Clorox effected differently the seeds. At 30% Clorox maximum germination was observed by the variety Galaxy-01 (16.6625%). Chakwal-50 (12.5%) and Borlog-14 (12.5%) showed the same germination at 30% Clorox. NARC-09 (8.25%) and Millet-11(8.25%) showed the same results and Pakistan-13 (0%) showed no germination at 30% Clorox. Maximum germination at 50% Clorox was shown by the variety Galaxy-01 (29.1625%) and Millet-11 (29.1625%). NARC-09, Pakistan-13 and Chakwal-50 showed 20.825%, 20.825% and 16.6625% germination respectively. Less germination was shown by the variety Borlog-14 (4.1625%). At the concentration of 70% Clorox the maximum germination was shown by the variety Borlog-14, which is 37.5%. Millet-11 showed 25% germination. NARC-09 and Pakistan-13 showed no germination at 70% Clorox. Less germination was recorded by the variety Galaxy-01(12.5%). The results indicated that all varieties behaved differently at different levels of concentrations. It is concluded that for sterilization of wheat seeds the 50% Clorox is better than other concentrations used.

Keywords: Galaxy-01 (16.6625%), NARC-09 (8.25%) and Millet-11(8.25%).

[Full Text Article]



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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database