EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

STUDY COMPARATIVE ANTI-MICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF FLOWERS OF CALOTROPIS GIGANTEA AND CALOTROPIS PROCERA

P. B. Kadam*, D. S. Kachare and K. T. Sul

ABSTRACT

Ayurveda is the oldest medicine system and includes numerous medicinal plants. Medicinal plants are massive source of biologically active compound and offering broad range of activity. The present study was aimed to evaluate and compare antimicrobial activity of methanolic extract of flowers of Calotropis gigantea and Calotropis procera which is also known as Rui. Its antimicrobial activity was performed by using gram negative bacteria Escherichia coli; gram positive bacteria Bacillus subtilis and fungi Aspergillus niger by cup plate method. Streptomycin and Itraconazole was used as a standard and to compare the antimicrobial activity of plants on pathogens. The antimicrobial activity was measured by different concentrations of methanolic extract of flowers of Calotropis gigantea and Calotropis procera with its minimum dose (20μg/ml) and maximum dose (100μg/ml) and Streptomycin (100μg/ml) and Itraconazole (100μg/ml). Antimicrobial activity was evaluated by measuring the zone of inhibition in millimeters. It can be concluded that methanolic extract of Calotropis gigantea and Calotropis procera was found to have antimicrobial activity against gram positive Bacillus subtilis; gram negative E. coli and fungi Aspergillus niger. It also indicated that methanolic extract of pants are potentially good for antibacterial resistant bacteria therapy.

Keywords: Antimicrobial activity, Soxhlet extraction, Ultrasonication, cup plate method.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia