FORMULATION AND CHARACTERIZATION OF ANNONA SQUAMOSA LINN LEAF EXTRACT NANOSUSPENSION FOR ANTIDIABETIC ACTIVITY

E. Abraham Theodore*, P. Indhu, Dr. Barish and Dr. R. Venkatanarayanan

ABSTRACT

Currently, nanodelivery systems are an area of intense focus for the delivery of bioactive compounds to ensure effective treatment of various chronic diseases. Annona squamosa L. has belonging to the family Annonaceae and the leaves have shown to possess larvicidal, hepatoprotective, anti-bacterial, wound healing, anti-diabetic, etc,. Methanolic extract of Annona squamosa L. leaves, a poorly water-soluble drug is formulated as nanosuspension by nanoprecipitation method using Tween 80 and Span 80 as polymers in different ratio, for the improvement of solubility and bioavailability. The Annona squamosa L. nanosuspension were characterized by particle size, zeta potential, drug entrapment efficiency are 223.2nm, -12.4mV, 81% respectively. In vitro drug release and drug kinetics were also studied. The optimized formulation was found to follow zero order release pattern which was revealed by the linearity shown from the plot of Time vs Concentration. It was concluded that Annona squamosa L. nanosupension facilitate higher cellular penetration and possess high bioavailability and sustained release.

Keywords: Nanosuspension, Annona squamosa L., Nanoprecipitation, Solubility and bioavailability.