ACTIVITY OF ANTI-LACTIC ACID BACTERIA ISOLATED FROM MILK AND MILK PRODUCTS AGAINST PATHOGENIC BACTERIA OF HUMANS

Mohamed Farag Elbreki, Suad Abdullah Noureddine, Abdullah Shebah* and Abdulbasit Naiel

ABSTRACT

The study included isolation and diagnosis of types of lactic acid bacteria from animal milk and local products, and the detection of the inhibitory ability of their metabolites against some types of pathological bacteria, where types of lactic acid bacteria were isolated from 25 samples that included fresh animal milk (cows, camels, goats) and a dairy product traditional Libyan (Laban Rayeb).126 isolates were obtained from lactic acid bacteria. They were identified on the basis of the common properties of the lactic acid bacteria based on the cultural, microscopic, and biochemical tests. The identity of some isolates was also confirmed by using the system: API 50CHL, Where possible Diagnosis of 4 isolates belonging to Lb.fermentum And 3 isolates belong to Lb.bulgaricus. And 2 isolates belong to Lb.casei And 2 isolates belong to Lb. salivarius And 2 isolates belong to Lb. acidophilus And 1 isolates belong to Lb. plantarum And1 isolates belong to Lb. lactis. The efficacy of the isolates filtrate in inhibiting the growth of some types of pathogenic bacteria was tested by using the test of disks saturated with the filtrate of the selected isolates. It was found that lactic acid bacteria filtrates have different inhibitory efficacy against some types of pathological bacteria, as the diameter of the inhibition zones ranged between 3-35 mm, Six of these isolates of the LAB which includes (Lb96, Lb5, Lb131, Lb151, Lb155 and Lb114) showed the highest inhibiting activity.

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