OVEREXPRESSION OF LINC00319 PROMOTE PROLIFERATION, MIGRATION AND INVASION OF HCC.

Peter Kisembo, Christian Cedric Bongolo, Souraka Tapara Dramani Maman and Jian-Cheng Tu*

ABSTRACT

Objective: Liver cancer is the second largest cancer death cause in the world. In order to improve the survival rate of patients with liver cancer, it is important to study the genes related to tumour metastasis and find new therapeutic targets. More and more studies have shown that long noncoding RNA plays an important role in the regulation and development of tumours. Long intergenic non-protein coding RNA 319 is a new long noncoding RNA, and its role in HCC has never been studied. Methods: The expression of linc00319 in hepatocytes was detected for the first time. The expression level of linc00319 was detected by real-time fluorescence quantitative PCR (QRT PCR), and then the appropriate hepatocytes were selected for stable modulation and transfection. Cell proliferation and migration were detected by CCK-8 and Transwell migration test respectively. Results: Compared with normal hepatocyte line (L-02), linc00319 was significantly increased in hepatocytes (P < 0.001), and its overexpression promoted the proliferation and invasion of hepatocytes. Conclusion: linc00319 plays an important role in the growth and development of hepatoma cells.

Keywords: HCC; long non-coding RNA; proliferation; invasion; migration.