EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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Abstract

A SNP BARCODE TO INFORM GENETIC VARIATION AND EVOLUTION PLASMODIUM FALCIPARUM MALARIA IN MALI

Abdoulaye Diawara*, Aoua Coulibaly, Cheickna Cisse, Mamadou Wele, Jeffrey Shaffer, Seydou Doumbia, Talib Yusuf Abbas and Mahamadou Diakite

ABSTRACT

Background: Despite of considerable advances in malaria control strategies, malaria continues to kill countless numbers of kids, mainly in sub-Saharan African countries. Mali is listed among the ten countries most suffering from malaria within the world in keeping with the quantity of cases and deaths... Plasmodium falciparum parasite is related to the foremost severe cases of malaria, especially in children. Malaria reports increasingly state drug resistance with severe cases, so investigating the genetic diversity and evolutionary history of Plasmodium falciparum are going to be crucial to understanding the evolution and variation of malarial drug resistance, and within the explore for potential vaccines. During this study, 186 samples were obtained from two sites in Mali: Koila Bamana in near of the town of Ségou and Nioro-du-Sahel situated within the Sahelian region. Methods: The 27-SNP barcode assay and phylogenetic tree (maximum likelihood method) were generated to research the variety and evolutionary relationship of the Plasmodium falciparum parasite population of 80 isolates in two varying patterns of malaria transmission in Mali. Excluding barcode sequences containing over four (4) missing SNPs, Minor allele frequencies (MAF), Genetic differentiation (GST= Genetic statistic), Average Minor allele frequencies (AMAF), the principal component analysis (PCA) were determined. Results: Fifty-seven of 80 isolates (71.3%) provided sufficient data quality for meeting the inclusion criteria of barcode. The parasite populations displayed a high degree of intra-population genetic diversity (MAF >1), the inter-population diversity was relatively low (AMAF = 0.34 and = 0.28 for Koila Bambara and Nioro du Sahel respectively) consort with barcode π value respectively 0.43 and 0.44 for Koila Bambara and Nioro. The populations failed to differ with relevance genetic diversity (GST=0.03). In both populations, the PCA sustained the genetic similarities of P. falciparum. Analysis of the phylogenetic tree revealed several groups of P. falciparum genetically close, which could have originated from Nioro du Sahel. Conclusions: The genetic diversity of P.falciparum populations is a major is a major consider in the parasite's ability to adapt to changes in its environment thus the measures observed here for Koila Bambara and Nioro du Sahel may partially explain the rising drug-resistance and growing severe cases in those regions in Mali.

Keywords: SNP, Barcode, Evolution Malaria, Plasmodium falciparum, genetic diversity, Mali.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI )