STUDY OF ADENOSINE DEAMINASE IN BREAST CANCER

*Dr. Sucheta Ghule (MD Biochemistry) and Dr. B. B. Murhar (MD, Biochemistry)

ABSTRACT

Introduction: There are numerous growing evidences for the increased oxidative metabolism in cancer cells which is reflected by the enzyme activities of the cancerous cells. Increased metabolic activity alter the normal DNA and RNA metabolising enzymes like Adenosine Deaminase (ADA) along other ubiquitous enzymes like Alkaline Phophatase, the levels of which can be used for diagnosis and prognosis. Material And Methods: In present study 50 cases of breast cancer were subjected for the estimation of serum activities of adenosine deaminase(ADA), heat stable alkaline phosphatase, and alkaline phosphatase. The 50 cases of breast were divided in four sub-groups of stage I to stage IV according to four stages of TNM classification. The results were compared with enzyme activities in normal age, sex matched healthy adults as controls. The enzyme activities was compared between operated and unoperated cases and also in cases with metastases and those without metastasis. Stastisical Analysis: The statistical analysis was done by using students “t” test for cases and controls. Annova was applied to compare the enzyme activities in different stages. Result: The study revealed a highly significant elevation of enzymes ADA, in breast cancer cases(p <0.001)as compared to controls with stage wise increase in enzyme activity. Presence of heat stable alkaline phosphatase was found in 98% of cancer patients. Activities of ALK though were higher in cancer patients the increase was not statistically significant(p=0.5). The values of ADA were correlating well with the clinical stage of disease unlike alkaline phosphatase. Conclusion: The result of study indicates the increased activities of of ADA in breast cancer as well it‟s correlation with the clinical stage of disease. The re-expression of HsALK in breast cancer patients was found to be significant.

Keywords: ADA, ALK, Breast cancer, HsALK.