PHYSICOCHEMICAL ANALYSIS, QUALITATIVE AND QUANTITATIVE INVESTIGATION OF ATROPINE IN MEDICINAL PLANT ATROPA BELLADONNA

Mahrukh Malik*, Shahzad Hussain1, Naila Sajjad, Sara Nazir, Muhammad Usman Gondal, Javeria Arshad Malik

ABSTRACT

Atropa belladonna, commonly known as belladonna or deadly nightshade. The plant contains tropane alkaloids, including atropine, scopolamine, and hyoscyamine, which are used as medicinal, herbal and homeopathic remedies. These alkaloids are highly dose dependent. A greater understanding of this fact is critical in order to develop better treatment strategies, therapies, regulations, education of at-risk populations, and a more cohesive paradigm for future research. This research offers an integrated view of the homeopathy of Atropa belladonna. Present investigation is conducted to evaluate the physiochemical measures of accessible mother tinctures of Atropa Belladonna. Mother tinctures were collected from five manufacturers in Pakistan and evaluated for physiochemical parameters, % alcohol contents, Weight on dry/ml, % Non-volatile Matter, pH and for identification of atropine. World synchronization to follow WHO specific guidelines for herbal products standardization are of greatest need and to endorse regulation to control herbal market to adapt WHO guideline.

Keywords: Herbal drugs, Standardization, Quality control, Atropa Belladonna, atropine,uv-visible spectrophotometer, FTIR, HPLC, Atomic absorption spectrophotometer.