POTENTIAL HYPOGLYCEMIC EFFECT OF BINAYUYO (ANTIDESMA GHAESEMBILLA, FAMILY: PHYLLANTHACEAE) LEAVES CRUDE EXTRACT – ALLOXAN INDUCED HYPERGLYCEMIC INSTITUTE OF CANCER RESEARCH (ICR) MICE

Shalom James Apura, Ricarianne Coleen David, Cyrus Jefferson Faa, Nerick Garrido, Daniele Roman Joson and Anthony Marin*

ABSTRACT

Plants have been one of the most used natural resources for medical purposes especially in Asia. The world’s population (75-80%) relies on plants for their primary health care according to the World Health Organization (WHO). Perceiving the enormous potential, they offer, therapeutic plants have been continually utilized in defining new medications. This study aimed to determine the potential hypoglycemic effect of Binayuyo leaves crude extract (Antidesma ghaesembilla, Family: Phyllanthaceae) using Alloxan induced hyperglycemic Institute of Cancer Research (ICR) mice. Binayuyo tree grows with a thick crown that could be measured up to 20 meters tall. Its fruit has a touch of purple. Other parts of the tree were accounted to be used for food and medication. The researchers used the ethanolic crude extract of Binayuyo leaves to be tested for the potential hyperglycemic effect. The crude extract was put to rotary evaporator to discard excess alcohol. After the process, the product was used to mice. The study employed a total of 36 ICR mice wherein 6 mice were allocated per group. There were five groups in the study; Distilled water as the negative control, insulin as the positive control, 500 mg Binayuyo extract, 750 mg Binayuyo extract, and 1000 mg Binayuyo extract. Observations were done in a four-hour period. Results showed that distilled water does not significantly lower the glucose level in 4 hours of observation. Binayuyo shows a potential hypoglycemic effect on the fourth hour of observation compared with the glucose level of post-alloxan. Binayuyo at 750 mg and 1000 mg of concentration has no significant difference with insulin in lowering glucose level.

Keywords: Antidesma ghaesembilla; Alloxan induced; ICR Mice; Binayuyo Leaves; Potential Hypoglycemic Effect.