ANALYSIS OF ANTIOXIDANT AND LIPID PEROXIDATION EFFECT OF ORGANIC EXTRACTS OF CALYCOPTERIS FLORIBUNDA (LINN) IN RAT FED WITH HIGH FAT DIET

Bhuvaneswari Santharam*, Ganesh Punamalai, Soranam Ramiah, Vengadachalapathy Lakshmanan and Packia Lekshmi Narayana Chellaiya Johnson

ABSTRACT

The aim of this study was to investigate effect of various extract of Calycopteris floribunda on the lipid peroxidation and invivo antioxidant systems in tissue (Liver, Heart and Aorta) of high fat fed rats. High fat diet rats showed significantly (P < 0.001) reduced the levels of tissue enzymatic antioxidant and non enzymatic antioxidant (Glutathione). The level of Thiobarbituric reactive substances (TBARS) are elevated in HFD rats (group II) When compared with control rats (Group I). After administration of ethyl acetate extract of Calycopteris floribunda in high fat diet rats showed significantly (P < 0.001) increased the levels of antioxidant enzymes (CAT, SOD, GPx, GR) and non enzymatic antioxidant glutathione (GSH, CAT, SOD, GPx, GR) and lowered the concentration of TBARS when compared with other two extracts. The ethyl acetate extract of Calycopteris floribunda in high fat diet rats were found reduced the concentration of TBARS (a measure of lipid peroxidation) than that of HFD rats (Group II). In conclusion, ethyl acetate extract of Calycopteris floribunda could be an option to enhance the supply of antioxidants and to safeguard against oxidative stress and thereby preventing the formation of atherosclerotic plaques.

Keywords: Calycopteris floribunda, Antioxidant, High Fat diet, Rats, Lipid peroxidation.