COMPARATIVE PHYTOCHEMICAL STUDY, ANTIOXIDANT CAPACITY AND ANTIMICROBIAL ACTIVITY OF DIFFERENT PROPAGATED CALLUS OF ECHINACEA PURPUREA AGAINST ITS LEAF EXTRACTS

M.E.S. Hassan*, K. F. Taha, I. A. Ibrahim, M. Bekhit, A. Ibrahim, S. E. Talat and M. Almahdy

ABSTRACT

Biotechnology has revolutionized the field of biological sciences worldwide. Importance of technological advances has created a renewed interest about its applications. The aim of this study is to use the biotechnology for propagation of Echinacea purpurea callus with high value secondary metabolitesin Egypt for medicinal use. In this work we evaluate total polyphenol, flavonoids and polysaccharides contents of Echinacea purpurea propagated callus using different media againstitsleaf extract. Also determined the most important potential active compounds, caffeic acid derivatives, as caffeic acid, chlorogenic acid and chichoric acid in all tested extracts. Investigate their antioxidant capacity and antimicrobial activity as a comparative study. The results showed that propagated callus (7) using 1.0 auxin has the highest total ployphenols and flavonoids content while callus (6) using 0.50 2,4-Dichloro phenoxy Acetic Acid (2,4D) +0.25 kinetin (k) has the highest total polysaccharides content. Chicoric acid in Echinacea extract was 22 mg/g and in callus (7) was 9.6 mg/g while chlorogenic acid was 12.25 mg/g in callus (7) and 8.5 mg/g in Echinacea extract. Echinacea extract showed the highest antioxidant activity followed by Callus (7). All tested extracts have antimicrobial activity. It could be concluded that the possibility of propagation of Echinacea purpurea secondary metabolites using biotechnology with valuable activity for medicinal use.

Keywords: Biotechnology, Echinacea purpurea callus, flavonoids, chichoric acid, antioxidant activity.