DETECTION AND QUANTITATION OF HIGH RISK HUMAN PAPILLOMAVIRUS, HRHPV 16 AND 18 IN TISSUE OF INDIAN WOMEN WITH CERVICAL CANCER: A CASE CONTROL STUDY

Pushpendra D. Pratap, Syed Tasleem Raza*, Ghazala Zaidi, Shipra Kunwar, Mark Rector Charles, Ale Eba and Muneshwar Rajput

ABSTRACT

Background: Cervical cancer is the second most common cancer in women worldwide. High-risk infection with HPV type 16 or type 18 is the most important risk factor associated with the development of cervical cancer. Objective: The present study was carried out with an aim to investigate the presence and type of HPV infection in cervical cancer cases as well as controls and to determine the association of HPV positivity with various patient and lesion characteristics. Material & Methods: A total of 96 histopathological confirmed cases of cervical cancer (CC), and 100 control samples with matched aged groups were enrolled in the study. Specimens were obtained from the site of lesion. All the samples were subjected to histopathological analysis and detection of hr-HPV-16 and 18 was done by using real-time polymerase chain reaction (RT-PCR). Results: Majority of cases from upper middle social class (91.67%) and (8.33%) from lower middle class while 82% and 12% in controls respectively. Most of the cases were Stage II (57.30%), and Stage I (21.87%), while Stage III (15.62%), and Stage IV (5.21%) respectively. Size of the tumors were 2–4 cm2 (59.38%), while <2cm (19.79%) and >4cm (17.71%). Majority had Keratinizing SCC (85.42%). Most of the cases were HPV 16 positive (65.63%) followed by HPV 18 positive (18.75%) respectively. Conclusions: The present study highlighted the fact that presence of HPV 16, 18 associated with development and progression of cervical cancer.

Keywords: Human papillomavirus (HPV), cervical cancer (CC), real-time polymerase chain reaction (RT-PCR).