EFFECT OF POLLUTANTS ON FISH AND AMPHIBIAN WITH VARIOUS HISTOCHEMICAL CHANGES
*Dr. Mukesh Kumar Napit
ABSTRACT
In present study, the comparative study on midgut trehalase in fifth instars larvae of bivoltine races CSR2, CSR2×4 and CSR4 were done. The maximum activity of trehalase showed at pH 5.5 in all the races at maximum temperature 500C in CSR2 and CSR2×4 while in CSR4 at 450C. The digestion time required for 30 minutes in race CSR2 and CSR2×4 and 20 minutes for CSR4.The Km values calculated from the graph. The Km values for CSR2, CSR2×4 and CSR4 were 10.57x10-3M, 2.57 x10-3M and 0.77x10-3M respectively. It indicates that CSR4 showed maximum efficient trehalase activity as compared to other two races.
Keywords: Midgut trehalase, Bivoltine, Silkworm.
[Full Text Article]
[Download Certificate]