VALIDATED HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF LOPINAVIR AND RITONAVIR IN TABLET DOSAGE FORM
S. H. Alhat*, H. P. Alhat and S. V. Joshi
A high performance thin layer chromatographic method has been developed for the simultaneous determination of
lopinavir and ritonavir from tablet dosage form. Separation was performed on aluminum HPTLC plate (20×10cm)
precoated with silica gel F254 HPTLC plates as stationary phase and the mobile phase consisting of toluene, ethyl
acetate, methanol, formic acid (6:4, 4.5:0.5:0.5v/v/v/v) and wavelength of detection 254nm was used. After
development, plates were observed under UV light. The detector response was linear in the range of 2μg/spot -
12μg/spot and 2 μg/spot - 6 μg/spot for lopinavir and ritonavir respectively. The developed method was validated
as per ICH guidelines. The validated lowest limit of detection was 0.004827 μg /spot and 0.003369 μg /spot
whereas lowest limit of quantification was 0.014627 μg /spot and 0.010208μg /spot for lopinavir and ritonavir
respectively. The described method has the advantage of being rapid and easy. Hence it can be applied for routine
quality control analysis of lopinavir and ritonavir from pharmaceutical preparation and stability studies.
Keywords: Lopinavir, Ritonavir, HPTLC, Validation.
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