DESIGN AND OPTIMIZATION OF BIOETHANOL PRODUCTION FROM FRUIT AND VEGETABLE WASTES USING SACCHAROMYCES CEREVISIAE
Suganya Ramar* and Gayatri M.
The universal energy demand is contentiously increasing day by day because of rapid rise in world population and industrialization. Here is the main objective is to overcome this energy crisis by using biofuel as an alternative energy source. Bio ethanol is most often used as a biofuel additive for gasoline and also used as an alternative to conventional fossil fuels. Now-a-days bioethanol production from fruit and vegetable wastes are very easy process and also has low cost. In this study, raw materials are subjected to acid hydrolysis, which convert the wastes into fermentable sugar and Saccharomyces cerevisiae commonly known as baker’s yeast used for fermentation process. The focus of this present study is to investigate the best carbon and nitrogen source and optimization of medium composition for maximum production of bioethanol. The best nitrogen source was selected by one variable at a time approach. The greatest nitrogen source was yeast extract used to maximize the bioethanol production. The Plackett-Burman design was used for identification of significant variables that are pH, temperature and inoculum’s size. The composition level for each significant variable was determined using Response Surface Methodology. From central composite design, inoculum’s size (4 ml/100ml), pH (6), temperature (42.5 o C) are the optimize values for bioethanol production and that confirmation location gives 95% confidence level in point prediction. Based on the optimization process 90% of ethanol yielded from wastes. This was analyzed using dichromate assay.
Keywords: S. cerevisiae, fermentation, bioethanol, PB design, CCD.
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