PHARMACOLOGICAL SCREENING FOR ANTI-INFLAMMATORY, ANALGESIC, AND CNS DEPRESSANT ACTIVITIES OF THE METHANOL AND ETHYL ACETATE FRACTIONS OF STEREOSPERMUM PERSONATUM (HASSK.) CHATTERJEE LEAVES IN MICE

Luthfun Nesa*, Md. Akherul Islam, Mohammad Amzad Hossain, Md. Monirul Islam, Asma Kabir and Mohammad Sirajum Munir

ABSTRACT

Aims: The study was carried out to assess anti-inflammatory, analgesic and CNS depressant activities of methanol & ethyl acetate fractions of Stereospermum personatum leaves. The methanol and ethyl acetate fractions (200mg/kg and 400mg/kg dose) were ingested to determine the acetic acid-induced writhing, formalin induced licking and biting, carrageenan induced hind paw edema and CNS depressant activity by open field and hole cross test. The both fractions (400mg/kg) exhibited moderate analgesic activity (57.55% and 56.1%) against acetic acid induced pain compared to standard (83.02%). Though the both fractions (400mg/kg) showed same analgesic effect (54.57% and 56.43%) at early phase but only ethyl acetate fractions (400mg/kg) is effective (58.21%) at late phase of formalin induced compared to standard (59.70%). The both fractions (200mg/kg and 400mg/kg) exhibited significant anti-inflammatory activity at 1st h to 4th hours. The two fractions (200mg/kg and 400mg/kg) showed significant (*p<0.05) depressant activity at 30min to 120min in the hole cross and open field test. This study observed that the effect of methanol and ethyl acetate fractions of Stereospermum personatum leaves (400mg/kg) were almost similar but only 400mg/kg dose has shown moderate analgesic, significant CNS depressant action and mild anti-inflammatory activity

Keywords: Stereospermum personatum, Analgesic, acetic acid, Formalin, anti-inflammatory, CNS Depressant