IN-SILICO STUDY: PROTEIN STRUCTURE PREDICTION, COMPARISON AND COMPARATIVE STRUCTURAL ANALYSIS BY ALIGNMENT OF SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 NUCLEOCAPSID PROTEIN (SARS-COV-19 N PROTEIN) AND DIFFERENT ORGANISM’S HOST WITH ACE2 PROTEIN

Krushna K. Dishware*, Dr. Faiyaz K. Shaikh and Dr. Sanjay N. Harke

ABSTRACT

Human beings are presently experiencing a serious global public health issue that is Coronavirus disease 19 (COVID-19). The World Health Organization (WHO) on March 11, 2020, has declared the novel Coronavirus disease 19 (COVID-19) outbreak a global pandemic. Coronavirus disease 19 (COVID-19) has a single - stranded RNA genome. There are seven strains of human Coronavirus (CoVs) ,out of those three strains are highly pathogenic (SARS-CoV, MERS-CoV, 2019-nCoV), which causes an endemic of severe Coronavirus (CoV) disease. The most important four structural proteins of COVID-19 are S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The nucleocapsid protein is one of the core components of the SARS-CoV-2. Nucleocapsid protein alone is able to form the capsid. In this present work, we have predicted tertiary structure of human, Bats (Pteropus vampyrus), Mus musculus with ACE2 protein with the help of Swiss model (automated protein structure homology-modelling server). Protein structure alignment and comparison of different organism's host with ACE2 protein and SARS-CoV-2 Nucleocapsid protein using PyMol (3-Dimensional structure visualization Tool). We also performed Superimposition of proteins, comparative structural analysis. Calculate their RMSD (Root Mean Square Deviation) and RMS score.

Keywords: COVID-19, SARS-CoV-2 Nucleocapsid protein, Structure prediction, Structure alignment and comparison, comparative structural analysis.