ANTIOXIDANT ACTIVITY OF HAIR TONIC FROM ETHANOL EXTRACT, ETHANOL FRACTION, AND CHLOROFORM-METHANOL FRACTION OF SECANG WOOD (CAESALPINIA SAPPAN L.)

Dina Yuspita Sari*, Ratna Widyasari and Verlia Tesya Artari

ABSTRACT

Secang wood (Caesalpinia sappan L.) is a plant of the Caesalpiniaceae family that is commonly found in Indonesia. Secang wood contains brazilin compounds which have antioxidant benefits. This study aims to determine the IC50 value of each hair tonic formula with the active substance of ethanol extract, ethanol fraction, and chloroform-methanol fraction of secang wood (Caesalpinia sappan L.). The simplicia of secang wood was macerated using 96% ethanol, then partitioned using n-hexane and 96% ethanol. The ethanol fraction was then applied using vacuum column chromatography with silica gel 60 as a stationary phase using chloroform, chloroform: methanol (5: 1) and methanol as eluent. Detection of the fraction obtained using a UV lamp at λ 254 and 366 nm. The fractions with the same smudge pattern are then collected. The ethanol extract, ethanol fraction, and chloroform-methanol fraction of secang wood were then formulated into a hair tonic formula. Antioxidant activity test of hair tonic formulas determined using the DPPH method. The results showed that hair tonic formula using ethanol extract, ethanol fraction, and chloroform-methanol fraction of secang wood each had IC50 values of 700.859 ppm, 505.169, and 855.930 ppm (weak antioxidant activity)

Keywords: antioxidant activity test, DPPH method, hair tonic, secang wood (Caesalpinia sappan L.)