( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



Tchoupou-Tchoupou E. C.*, Osseyi E. G., Ndofor-Foleng H. M. and Onyimonyi E. A.


The inappropriate and irrational use of antibiotics in poultry industry has led to the emergence of resistant microbial populations such as Salmonella typhimurium, which is responsible for 26% of cases of enterocolitis in humans. In the fight against this bacterium, Allium sativum (garlic) would be an effective alternative. This is because several studies claim the effectiveness of garlic in the prevention and treatment of bacterial diseases. To determine the in vitro antibacterial activity of hexane extract of garlic on the growth of Salmonella typhimurium, different concentrations of 50, 100, 200, 400 and 800 mg/ml of extract were used. The antibacterial activity was evaluated using agar well diffusion test method. Macro-titer tubes were used to determine the minimum inhibitory concentration (MIC). The minimum bactericidal concentration (MBC) was obtained by streak plate method. Ciprofloxacin was used as a positive control and distilled water as a negative control. The concentrations of 200, 400 and 800 mg/ml showed inhibition zones with diameters ranging from 14 to 24 mm; 29 mm for ciprofloxacin and 0 mm for distilled water. The MIC were between 31,25 to 62,5 mg/ml while MBC was 250 mg/ml. The present work concludes that, to fight Salmonella typhimurium, hexane extract of garlic can be used as a potential alternative to antibiotics.

Keywords: Garlic, Medicinal plant extract, Disc diffusion technique, Macro dilution technique, Salmonella typhimurium strains, Minimum inhibitory and minimum bactericidal concentration.

[Full Text Article]



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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database