RP- HPLC METHOD FOR ESTIMATION OF PSEUDOEPHEDRINE HYDROCHLORIDE IN BULK AND TABLET
Rahul K. Godge*, Nachiket S. Dighe and Rohini R. Gore
A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the estimation of Pseudoephidrine hydrochloride from pharmaceutical formulation by using internal standard Chlorpheniramine maleate. The method was carried out on a HiQ sil C18 W (250mm x 4.6mm) column, with a mobile phase consisting of Acetonitrile: Methanol : Phosphate buffer (45:40:15 v/v) at a flow rate of 1.5 ml/min. Detection was carried out at 265 nm. The retention time of Pseudoephidrine hydrochloride and Chlorpheniramine maleate were 2.508 and 3.300 min. respectively. The developed method was validated in terms of accuracy, precision, linearity, Limit of detection, Limit of quantitation. The proposed method can be used for estimation of drug in dosage form for routine analysis.
Keywords: Pseudoephedrine hydrochloride, Chlorpheniramine maleate, RP-HPLC.
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