DPPH AND ABTS RADICAL SCAVENGING ACTIVITY IN KALOPANAX SEPTEMLOBUS

Man Kyu Huh*

ABSTRACT

The aim of this study was to investigate the antioxidant activity of several solvent extracts including water from leaves, stems, and spines of Kalopanax septemlobus. The antioxidative activities of the extracts were determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging methods. DPPH scavenging activity of water extract for leaves evaluated at 1.0 mg/ml was 65.5% and that of butanol was only 51.0% at same concentration. The antioxidant properties of leaf extract were high. Stem and spine effects were not great levels. There was no significant difference among three tissues (p>0.05). DPPH and ABTS scavenging ability at two concentrations (0.5 mg/ml and 1.0 mg/ml) showed significant differences in all tissues (p<0.01-0.001). The Trolox (positive control as ABTS) exhibited inhibitory activity against the ABTS cation of 44–91% at concentration ranges of 1.0 μg/mL. According to antiradical efficiency parameter, the results of DPPH and ABTS showed that the leaf, stem, and spine of K. septemlobus were low antiradical activity as an effectivenatural source of antioxidant.

Keywords: ABTS, DPPH, Kalopanax septemlobus.