EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

THE EFFECT OF VITAMIN E AS DIFFERENTIATION-LIKE FACTOR IN CML BLAST CRISIS K562 CELLS.

Shvachko L. P.*

ABSTRACT

Background: Chronic myelogenous leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene that arises from the reciprocal translocation t(9;22)(q34;q11). The molecular events underlying the transition from the chronic phase to the blast crisis are still poorly understood. The transcription factor C/EBP-alpha (CCAAT/enhancer-binding protein α) implicated as an inhibitor of cell proliferation and a master regulator of hematopoietc stem cell (HSC) myelopoiesis plays a critical role in granulocytic differentiation. The progression of CML to blast crisis is correlated with down-modulation of myeloid C/EBP-alpha transcription factor. Therefore, C/EBP-alpha may be considered as a putative target in differentiation therapies in myeloid leukemias as CML and AML. The aim of the study was to assess the potential of vitamin E as the possible inducer of C/EBP-alpha expression in BCR-ABL-positive chronic myelogenous blast crisis (BC-CML) K562 leukemic cells. Methods: RNA extracted from CML blast crisis K562 leukemic cells was converted to cDNA and real-time RT-PCR was performed using SYBR Green protocol with primers to C/EBP alpha and G-CSFR genes. Samples were cycled using the standard SYBR Green protocol on Q5 Bio-Rad StepOne qPCR instrument and analyzed using the comparative cycle threshold (CT) method to obtain relative mRNA expression. To analyze the modulation of expression of C/EBP alpha and G-CSFR, CML cells were incubated with 100 μM vitamin E. Results: We have not found detectable expression of C/EBP-alpha in CML blast crisis K562 cells. Upon 48-h culture with vitamin E at a dose of 100 μM, K562 cells expressed both C/EBP-alpha and G-CSFR (granulocyte colony-stimulate factor receptor) gene expression. Conclusion: Vitamin E restored the expression of C/EBP-alpha mRNA in CML blast crisis K562 cells. In this setting, G-CSFR expression in vitamin E treated K562 cells seems to point out to the activation to granulocytic differentiation. It should be further elucidated whether such effects of vitamin E on C/EBP-alpha transcription factor are direct or mediated through generation of indirectly due to antioxidant properties of vitamin E.

Keywords: C/EBP alpha and G-CSFR, CML cells.


[Full Text Article]

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing