STUDY OF ANTIMICROBIAL ACTIVITY OF LANTANA INDICA LINN. AGAINST SOME HUMAN PATHOGENS. AND ITS PHYTOCHEMICALS.

Shubha Gupta, Kumudini Indira Toppo, Deepak Karkun and Anil Kumar*

ABSTRACT

Medicinal plants are the wealthy source of the antimicrobial agent and curative. In the present study we carried out the antimicrobial effect and analysis of phytochemical constituents of different parts of Lantana indica Linn (Verbenaceae). Pathogenically significant bacteria (Salmonella typhi-MTCC-733, Staphylococcus aureus- MTCC-7443, Pseudomonas aeruginosa MTCC-7296, Mycobacterium tuberculosis-MTCC-300) and fungus (Microsporum canis-MTCC-2820, Epidermophyton floccossum-MTCC-613, Trichophyton rubrum –MTCC-296, Aspergillus candidus –MTCC-1989) were selected for the study. The extract of root, stem, leaf and flower of plant were prepared in methanol and aqueous solutions and different doses (25 μl, 50 μl, 100 μl, 200 μl, 300 μl and 400 μl) were selected for study by agar disk diffusion method. We found significantly effective antibacterial property of Lantana indica at concentrations of 25 μl to 400 μl against Pseudomonas aeruginosa and at concentration of 400 μl against Salmonella typhi and Staphylococcus aureus but found completely ineffective against Mycobacterium tuberculosis. The significantly effective antifungal activity was found only at 400 μl concentration against Epidermophyton floccossum and Trichophyton rubrum but found completely ineffective against Microsporum canis and Aspergillus candidus.The secondary metabolites of extract effective against microorganisms was evaluated and recognized as saponins, tannins, cardiac glycosides, alkaloids, flavonoids, steroids and terpenoids. The HPLC profiling was also performed and was identified as Lantadens A, B, C, D which is effective agent against above microorganisms.

Keywords: Antimicrobial activity, Medicinal plants, Aqueous extract, Methanol extract, Human pathogens, HPLC.