AN APPROACH ON SCREENING AND PRODUCTION OF L-GLUTAMINASE FROM ASPERGILLUS SP THROUGH SUBMERGED FERMENTATION
*Vandana Shree Jonna and Ann Catherine Archer
Enzymes as drugs have two important features that distinguish them from all other types of drugs. First, enzymes often bind and act on their targets with great affinity and specificity. Second, enzymes are catalytic and convert multiple target molecules to the desired products. These two features make enzymes specific and potent drugs that can accomplish therapeutic biochemistry in the body that small molecules cannot. These characteristics have resulted in the development of many enzyme drugs for a wide range of disorders. Totally fifteen Aspergillus sp were isolated from different soil samples from different areas in Bangalore. The isolated fungal strains were screened for L-glutaminase production by plate assay. Aspergillus sp V 08 were selected as best protease producer (1.02cm). The Aspergillus sp V 08 were employed for further production of L-glutaminase studies. The different pH (5), temperature (350C) and inoculum size (1.0 ml) were optimized and it showed 96 IU of L-glutaminase activity.
Keywords: L-glutaminase, submerged fermentation, thin layer chromatography and plate assay.
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