EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

ANTIFUNGAL ACTIVITY ON LASIODIPLODIA THEOBROMAE AND PHYTOCHEMICAL STUDY OF MITRACARPUS VILLOSUS AND MORINGA OLEIFERA FROM KISANGANI (D.R.CONGO)

Kwembe JTK., Onautshu D.O., Mpiana P.T.*, Bekaert B. and Haesaert G.

ABSTRACT

Lasiodiplodia theobromae is one of the fungal strains that attacks cocoa crops. This strain has become a critical threat to cocoa cultivation in some regions of the word including in the Kisangani region in the Democratic Republic of Congo. This work involved the identification, extraction and in vitro evaluation of the antifungal activity of the phytochemical groups of Mitracarpus villosus and Moringa oleifera on the strain of Lasiodiplodia theobromae. The aqueous, 95% ethanolic and ethereal extracts of M. oleifera are more antifungal (respective percentages of inhibition PI: 85.8; 77.8 and 77.4%) compared to those of M. villosus (71.4; 74.2 and 80.0%). The minimum inhibition concentration, MIC of the aqueous extract of M. oleifera is the lowest (20 mg/mL with a PI at the MIC of 85%) followed by that of the ethanolic extract, (25 mg/mL with a PI at 77% MIC). The chemical screening revealed the very abundant presence of tannins in the two plants and abundant presence of saponins in M. villosus. The extraction yields of tannins in M. villosus and M. oleifera are 16.91 and 21.25%, respectively, and that of saponins in M. villosus, 3.83%. M. villosus contains two kind of tannins (gallic and catechetical) when M. oleifera contains condensed tannins. M. oleifera tannins have a PI of 69.6% while those of M. villosus are 45.6%; both have PI lower than that of M. villosus saponins, 74.4%. Determination of active molecules from the tannins of M. oleifera and the saponins of M. villosus is undergoing.

Keywords: Lasiodiplodia theobromae, Mitracarpus villosus, Moringa oleifera, antifungal activity, cacao.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing