EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

MOLECULAR DETECTION OF EHRLICHIA CANIS IN DOGS IN EGYPT

Esraa MA Khamis, Shwikar MA Ahmed, Dalia Talat and Madiha Salah Ibrahim*

ABSTRACT

Canine Ehrlichiosis is a fatal tick-borne disease in dogs transmitted by brown tick called Ripicephallus Sanguinus. Canine monocytic Ehrlichiosis is an intracellular gram-negative bacterial infection with 3 clinical phases: acute, subclinical, and chronic. Clinically, the dog infected with Ehrlichia shows common symptoms as fever, sever emaciation, off food, redness of conjunctiva. Here, blood samples were collected from 46 dogs (37 suspected with Ehrlichia) and 9 cases normal control from 3 Governmental veterinary units and 3 private clinics (24 males and 22 females) in Egypt. Out of the 37 suspected cases, 15 were feverish, 7 were off food, 15 appeared emaciated, 15 showed thrombocytopenia, and one case had epistaxis and died within 72 hours. By Nested PCR for the 46 cases, 17 (37.0%) were positive, while, 22 (47.8%) cases showed morulae in the monocytes. Nested PCR is more sensitive and accurate test confirming E. canis infection, however, blood smear testing could be used as a cheap, rapid routine diagnosis for dogs infested with ticks. The current work is the second for the detection of E. canis 16S RNA gene by Nested PCR in Egypt, further surveillance studies are still required to monitor E. canis infection in dogs in Egypt.

Keywords: E. canis, dogs, morulae, Egypt.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing