RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ELBASVIR AND GRAZOPREVIR IN BULK AND PHARMACEUTICAL DOSAGE FORM
Vakati Jestavardhan* and V. Haribaskar
A simple, Accurate and precise method was developed for the simultaneous estimation of Elbasvir and Grazoprevir in Tablet dosage form. Chromatogram was run through Inertsil ODS C18 (250 x 4.6 mm, 5) Column. Mobile phase containing Methanol: Acetonitrile taken in the ratio 35: 65(pH 4.6 adjusted with OPA) was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 259 nm. Retention time of Elbasvir and Grazoprevir and were found to be 2.499 min and 2.998 min. respectively. %RSD of the Elbasvir and Grazoprevir were and found to be 0.5 and 0.8 respectively. % Recovery was obtained in the range of 98-102% for Grazoprevir and Elbasvir respectively. LOD, LOQ values obtained from regression equations of Grazoprevir and Elbasvir were 0.21, 0.78 and 0.09, 0.24 respectively. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Keywords: Elbasvir, Grazoprevir, RP-HPLC.
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