A BIOTECHNOLOGICAL APPROACH ON SCREENING AND PRODUCTION OF MANGANESE PEROXIDASE BY FUSARIUM SP FROM ARECA NUT HUSK
Nidhi K. V.*, Varsha B. S., Prajwal M., Sai Padma Shree G., Ananda H. V. and Prakruthi G.
The family of extracellular ligninolytic enzymes typically includes lignin peroxidases laccases, manganese peroxidases, versatile peroxidases and other accessory enzymes. Out of these enzymes MnP is thought to play the most crucial role in lignin degradation. Manganese peroxidase, an ubiquitous enzyme among ligninolytic fungi, is an extracellular heme-containing enzyme known to catalyze the oxidation of Mn2+ to Mn3+ in a reaction requiring appropriate manganese chelator. This enzyme catalysis the oxidation of Mn2+ to Mn3+, which in turn can oxidize a variety of phenolic substrates, including lignin model compounds Six Fusarium sp were isolated from soil samples from different regions in Bangalore. Isolated fungal strains were screened for Manganese peroxidase production by plate assay. Fusarium sp NPSV 01 were selected as best Manganese peroxidase producer (9 mm). The Fusarium sp NPSV 01 were employed for further fermentation kinetics studies through solid state fermentation by using areca nut husk. The different pH (6), temperature (300C) and inoculum size (1.25 ml) were optimized and it showed 0.381U/ml of manganese peroxide activity.
Keywords: Manganese peroxidase, solid state fermentation, Areca nut husk, Fusarium and Plate assay. phytoconstituents, isolation, characterization.
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